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Callus was induced from the petioles of scented-geranium (Pelargonium graveolense) in MS medium containing various concentrations of plant growth regulators. The highest frequency of more than 70% of callus was induced in 2 ㎎/ℓ NAA and 0.5 ㎎/ℓ BAP or 2 ㎎/ℓ 2,4-D and 0.5 ㎎/ℓ BAP combined treatment, while not in 2,4-D, NAA or BAP alone. When the callus was transferred to the MS medium containing 0.05 ㎎/ℓ NAA and 0.5 ㎎/ℓ BAP, the highest intensity of shoot formation, 14 shoots/callus, was induced after 5 weeks. The highest rooting was observed on hormone-free rooting media from the regenerated shoots after 3 weeks, indicating that the regeneration from geranium callus might be possible by changing the hormone ratios. Peroxidase (POD) specific activities in callus induced from 2 ㎎/ℓ NAA and 0.5 ㎎/ℓ BAP were higher than those of 2 ㎎/ℓ 2,4-D and 0.5 ㎎/ℓ BAP callus during the entire culture periods. POD isozyme C3 was the main cathodic POD isozyme expressed in NAA and BAP callus, while C1 was the main in 2,4-D and BAP callus. However, anodic POD isozymes, A1, A2 and A3 were expressed with similar activities in both hormone combinations.
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